Seney National Wildlife Refuge protects 95,212 acres of diverse wetland and upland habitats that support a wide variety of wildlife. The refuge consists of marshes, fens, and bogs; coniferous and northern hardwood forests; upland barrens and meadows; and a 25,000-acre Wilderness Area. Approximately 65% of the refuge is wetland. The refuge supports an array of both wetland and upland wildlife species such as bald eagles, ospreys, loons, otters, beavers, black bears,white-tailed deer, and wolves. More than 6,400 acres of open water are managed in 21 major pools.

Seney Refuge offers a range of programs, events, tours, facilities, and opportunities that make it "A Great Place to Watch Wildlife."

Five satellite refuges are managed from Seney: Kirtland's Warbler, Huron Islands, Michigan Islands, Whitefish Point, and Harbor Islands.